General Sorting Information

Machine

Aria II
(BSL-2 and BSL-2+)

Sony SH800 BSL-3 FACS Aria MoFlo DV-1

Laser wavelength

488/633/405/561 (350-BSL2 Aria only)

488/633/405/ 561

488/633/405/350

488/633/405

488/633/350

Nozzle sizes (µm) 70/85/100/130
70/100 70/100
70
80
Cell Concentration 1-2 x107 1-2 x107 1-2 x107 1-2 x107 1-2 x107
Sort Rate  1,000-25,000 cells/second 1,000-5,000 cells/second 1,000-25,000 cells/second 1,000-30,000 cells/second 1,000-12,000 cells/second
Operating Pressure (psi) 70/45/20/10
20 70/20
60
30

Running media - 1% FBS in any phenol-red-free media. HEPES can be added at a final concentration of 25mM to media to keep cells well buffered for all-day sorts, and/or to collection media.

Collection media - this is usually 20% FBS in whichever media the cells are happiest. This is diluted 4-5x and ends up being approximately 5% FBS.

Filtering :

All samples are filtered through 37µ mesh prior to running by core facility personel, however if you wish to filter the samples yourself refer to the ordering information below.

Sterile 12x75 mm tubes with filter caps are available; Becton Dickinson Cat. number 352235 or Fisher Cat. number 08-771-23

Nitex Nylon Filtration Mesh order from: ELKO Filtering Co, LLC. www.elkofiltering.com (Nylon 6/6 solid mesh roll 37µ opening size, 40" wide x 36" long) Item#: 03-37/24

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