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Useful Links

Educational and Technical Links:

Education

International Society for Advancement of Cytometry - ISAC
http://www.isac-net.org

New England Cytometry
http://newenglandcytometry.com

Purdue University Flow and Image Cytometry
http://www.cyto.purdue.edu

http://www.cyto.purdue.edu/class/

http://www.cyto.purdue.edu/flowcyt/educate.htm

http://www.cyto.purdue.edu/flowcyt/labinfo/labinfo.htm

Maeckerlab Weblog - Research and training materials for flow cytometry and immune monitoring.
http://maeckerlab.typepad.com

BD Biosciences Educational Programs Online

https://www.bdbiosciences.com/en-us/webinars

http://www.bdbiosciences.com/us/s/training/e-learning/e-learningcourses for beginners

http://static.bdbiosciences.com/training/diva6_course/index.html?_ga=2.221200155.225408873.1527001100-1298382617.1506082957 for Diva training

 http://www.bdbiosciences.com/us/applications/research/multicolor-flow/m/745795/resourcestools/tools

http://www.bdbiosciences.com/us/s/webinars/archives

Howard M. Shapiro, MD.:  Practical Flow Cytometry, 4th Edition (free ebook)
https://www.beckman.com/resources/reading-material/ebooks/practical-flow-cytometry

Tools

BD Product Selection Tools
https://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools
 

BD Biosciences Spectral Viewer  
https://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry/product-selection-tools/spectrum-viewer

Cytek Spectrum Viewer  
https://cytekbio.com/blogs/resources/tagged/tools

Thermo Fisher Spectra Viewer 
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectraviewer.html

FluoroFinder
Design Experiments for UMASS Flow Core's cytometers’ laser and filter configurations.
https://umassmed.fluorofinder.com

Block that Fc Receptor
http://www.innovexbio.com/fc_receptor_block.html

Why use FMO? 
http://expertcytometry.com/fluorescence-minus-one-fmo-control/

Live/Dead Staining
Dead cells bind antibody non-specifically and can exhibit a higher autofluorescense. Thus it is very important to exclude dead cells from your analysis - or sorted cells to improve the quality of your experiment. There are a wide array of options to choose from. They range from the classic dyes (PI, DAPI, Hoechst or 7AAD) to the newer fixable live/dead stain versions.

Below you will find a selection of companies providing these dyes as well as offering very good explanations on how those dyes work and give examples.

Thermo Fisher LIVE/DEAD Fixable Dead Cell Stains
LIVE/DEAD Fixable Blue stain - UV
LIVE/DEAD Fixable Violet stain - 405
LIVE/DEAD Fixable Aqua stain - 405
LIVE/DEAD Fixable Yellow stain - 405
LIVE/DEAD Fixable Green stain - 488
LIVE/DEAD Fixable Red stain - 488 or 561
LIVE/DEAD Fixable Far Red stain - 633
LIVE/DEAD Fixable Near-IR stain - 633

Biotium Live-or-Dye™ Fixable Viability Stains
Live-or-Dye Fixable Viability Stains
Live-or-Dye NucFix™ Red, a unique cell membrane impermeable dye that specifically stains the nuclei of dead cells, labeled covalently and does not transfer between cells after fixation.

Bio-Rad Live-Dead Exclusion Stains
VivaFix Fixable Viability Dyes
ReadiDrop 7-AAD
ReadiDrop PI
PureBlu DAPI
PureBlu Hoechst 33342

BioLegend Live-Dead Zombie Stains
Zombie Fixable Dyes
Helix NP NIR (ex/em - 640nm/660nm)
Draq7 (ex/em - 633nm/695nm)
PI (ex/em - 493nm/636nm)
7AAD (ex/em - 546nm/647nm)

Abcam live-and-dead-cell-assay
Live and Dead Cell Assay

Filtration recommendations
- Sterile 12x75 mm tubes with filter caps; by Corning - Fisher Cat. number 08-771-23
- Nitex Nylon Filtration Mesh order from: ELKO Filtering Co, LLC. (elkofiltering.com) Nylon mesh roll 37µ opening size, 40" wide, sold by the yard) Item#: 03-37/24 
- Miltenyi MACS® SmartStrainers  #130-098-458

Corporate – Instruments and reagents…

Abcam
http://www.abcam.com/

Amnis ImageStream Cytometer
http://www.amnis.com

Apogee
http://www.apogeeflow.com

BD Biosciences
http://www.bdbiosciences.com    

BeckmanCoulter
https://www.beckman.com/coulter-flow-cytometers

Biolegend
http://www.biolegend.com

BIO-RAD
https://www.bio-rad.com

Cytek 
https://cytekbio.com

Diatec Antibodies
http://www.diatec.com

eBioscience Antibodies
http://www.ebioscience.com

Evergreen Laser Corporation
http://www.evergreenlaser.com

Linscott's Antibody Directory
http://www.linscottsdirectory.com

MilliporeSigma
http://www.emdmillipore.com/US/en?bd=1

Miltenyi Biotec
http://www.miltenyibiotec.com/en/

Novus Biologicals
http://www.novusbio.com/novusknowsflow.html

Selleck Chemicals
http://www.selleckchem.com

Sony Biotechnology
https://www.sonybiotechnology.com/us/

Stratedigm
https://stratedigm.com/

VWR Antibody Selector
https://us.vwr.com/store/category/antibodies-iggy/3617058

Treestar (FlowJo) software
http://www.flowjo.com