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General Sorting Information

Machine

Aria II/II
Aria Fusion
(BSL-2 and
BSL-2+)

FACS Melody
BSL-2+

Sony SH800 
BSL-2+

BSL-3
FACS Aria IIu

Lasers
Parameters

(BSL-2+) 2B 6V 3R 2UV 4YG 

Aria Fusion


(BSL-2+) 5B 3V 3R 2UV 5YG 


"C" Aria


(BSL-2 only) 5B 2V 2R 

"S" Aria


 (BL2+) 2B 6V 3R 5YG 

"2" Aria

4B 5V 3R

488/633/405/561
(co-linear lasers)

5B 3V 4R 2UV 

Nozzle sizes (µm) 70/85/100/130
100 70/100 70/85/100
Cell Concentration 1-2 x107 1-2 x107 1-2 x107 1-2 x107
Sort Rate  up to 17,000 cells/sec. (70µm) up to 6,000 cells/sec. up to 5,000 cells/sec. up to 17,000 cells/sec. (70µm)
Operating Pressure (psi) 70/45/20/10
20 20/40 70/45/20

Nozzle choice - The diameter of your cells should be less than 30% of the diameter of the nozzle. Otherwise cells can be damaged and side streams can be diffused.  

Sort Rate - Each nozzle size has a limitation for the rate at which cells can be sorted efficiently. As a rule-of-thumb, divide the diameter of the nozzle by 5 to see the maximum rate at which the nozzle can sort. 

Running media - 1% FBS in any phenol-red-free media. HEPES can be added at a final concentration of 25mM to media to keep cells well buffered for all-day sorts, and/or to collection media.

Collection media - This is usually 20% FBS in whichever media the cells are happiest. This is diluted 4-5x and ends up being approximately 5% FBS.

Collection plates: Bring plates for collection with media pre-loaded in the wells. Do not fill wells above 1/2 maximum capacity to avoid spilling.

Filtering : All samples are filtered through 37µ mesh prior to running by core facility personel, however if you wish to filter the samples yourself refer to the ordering information below.

Sterile 12x75 mm tubes with filter caps are available; by Corning/Falcon #352235 - Fisher Cat. number 08-771-23

Nitex Nylon Filtration Mesh order from: ELKO Filtering Co, LLC. (elkofiltering.com) Nylon mesh roll 37µ opening size, 40" wide, sold by the yard) Item#: 03-37/24 

Miltenyi MACS® SmartStrainers  #130-098-458