Campus Alert: Find the latest UMMS campus news and resources at umassmed.edu/coronavirus

Search Close Search
Page Menu

UMMS Deep Sequencing Core Labs logo

The Deep Sequencing Core Facility offers high-throughput sequencing and library-building services for the UMass campuses and outside customers. The facility operates several types of Illumina next-gen sequencers, and is closely integrated with the PacBio Core Enterprise and the Molecular Biology Core Labs. See the Services page for detailed run options.

**If you have added a spike-in control to your sequencing library, please be sure to specify that information on the ticket. Controls for normalization such as "Active Motif" or other antibody spike-ins that are not removed often have poor base-balance/repetitive motifs, and can badly overseed on a flowcell.**

**Tickets and forms for sample submission can be found here.**

Pricing, Support, and Other Details
horizontal divider

For a sequencing pricing list, details on specific services, scheduling, or a quote for services, please email us here. Information about grant support letters can be found here.

Join Email List
horizontal divider

To be added to our email list for news and service updates, please contact us here.

Other Useful Information
horizontal divider

Drop-off locations:
Samples can be left in the labeled fridges at these locations
for 11:00 AM pickup each business day:
Biotech2 outside Suite 207
LRB 6th floor mailroom
ASC8-2058 mailroom
We are also available by appointment if you need to visit with us.
Our shipping address for off-campus customers is
Rose-Gordon Bldg. Rm. 141, 222 Maple Ave, Shrewsbury, MA, 01545.

Helpful Links:

For Illumina Library Building Notes, click here.

Puzzled about Multiplexing and Barcoding? Click here.

There is an updated list of oligo sequences for Illumina library construction. If you are a UMass investigator and wish to obtain a copy, please send an email to Nemo@umassmed.edu and request "the latest Illumina adapter sequences list".

For a letter of support for your grant, click here.

A list of frequently asked questions and answers is here.

The "How Long is THE QUEUE?" FAQ is located here.

We are updating our resources section. If you have a protocol or publication that you found useful, please share. If you have a request for something you think would be beneficial, please email us at DeepSequencingCoreLabs@umassmed.edu.

Pricing and Payment Policy
horizontal divider

Please email DeepSequencingCoreLabs@umassmed.edu to get a quote for your desired services.

Processing and analyzing a sample requires time and reagents. Payment for these services is the responsibility of the user submitting the sample and should be rendered in a timely fashion. In the event of a reagent or equipment failure, the samples will be rerun at the next possible opportunity at no additional charge.

Clients withdrawing samples prior to the analysis run will be charged a fee to recover QC assay costs. For the return of archived post-analysis samples, clients will be charged a delivery fee per sample.

Inclement Weather Policy
horizontal divider

In case of extreme and/or inconvenient weather, the machines will still be running but we may not be here for sample login or pickup. Please email us to arrange drop-off if the weather is questionable. The DSCL, MBCL, & PBCE are closed when UMassMed is on "inclement weather alert“.

UMMS PacBio Core logo linking to website

UMMS Molecular Biology Core Labs logo linking to website