Synergy H4 Micro Plate Reader

Synergy H4 Micro Plate Reader location: AS8-2028 status: in service reserve use
Multimode plate reader with dual injectors for time-critical reagent addition. Handles plates up to 1536 wells. Modes include luminescence, UV-visible absorbance, fluorescence intensity, time resolved fluorescence, fluorescence polarization, fluorescence resonance energy transfer (FRET), bioluminescence resonance energy transfer (BRET), and alphascreen. Dual monochrometers for excitation and emission provide high spectral resolution. Filter mode for high optical throughput
when required.

Specifications

General
Wavelength Selection	Hybrid Technology™ (patent-pending) Quadruple Monochromators and Filters / Dichroics
Detection method	Fluorescence, Time-Resolved Fluorescence, Fluorescence Polarization,AlphaScreen/AlphaLISA®, Luminescence, UV-Visible Absorbance
Read method	End point, kinetic, spectral scanning, well-area scanning
Microplate types	Monochromator system 1- to 384-well plates Filter system 1- to 1536-well plates PCR plates Compatible with Take3™ Multi-Volume Plate with 2 µL microspots
Temperature control	4° C above ambient to 65°, C + 0.5° C at 37° C
Shaking	Yes
Software	Gen5™ included; Control through USB or serial port
Automation	Yes
AlphaScreen/AlphaLISA
Light source	Tungsten Halogen Lamp
Sensitivity	100 amol of biotinylated-LCK-P peptide, 25 µL/well in 384-well plate
Dynamic range	>6 decades
Reading speed	2 minutes typical for 96-well plate
Detection system	Ultra Low Noise PMT
Absorbance
Light source	Xenon Flash Lamp
Wavelength selection	Monochromator
Wavelength range	230 - 999 nm, 1 nm increment
Bandpass	2 nm (230-285 nm), 4 nm (>285 nm)
Dynamic range	0 - 4.0 OD
Resolution	0.0001 OD
Pathlength correction	Yes
Monochromator wavelength accuracy	+2 nm
Monochromator wavelength repeatability	+0.2 nm
OD accuracy	<1% at 2.0 OD typical <3% at 3.0 OD typical
OD linearity	<1% from 0 to 3.0 OD typical
OD repeatability	<0.5% at 2.0 OD typical
Stray light	0.03% at 230 nm typical
Reading speed	96: 11 seconds 384: 22 seconds
Fluorescence Intensity
Sensitivity	Monochromators: Fluorescein 2 pM typical (0.2 fmol/well; 384-well plate) (Top) Fluorescein 2.5 pM typical (0.25 fmol/well; 384-well plate) (Bottom) Filters/mirrors: Fluorescein 1 pM typical (0.1 fmol/well; 384-well plate)
Light source	Tungsten Halogen Lamp High Energy Xenon Flash Lamp
Wavelength selection	Double grating monochromators (Top/Bottom) and, Deep blocking filters / dichroic mirrors (Top)
Wavelength range	Monochromators: 250 - 900 nm Filters: 200 - 700 nm (900 nm option)
Dynamic range	Monochromators: 5 decades Filters/mirrors: >6 decades
Bandpass	Monochromators: variable 9, 13.5, 17, 20 nm Filters: filter-dependant, from 5 nm to >100 nm
Detection system	Red shifted PMT for monochromator system Low Noise PMT for filter-based system
Reading speed	96: 11 seconds 384: 22 seconds 1536: 42 seconds
Luminescence
Sensitivity	10 amol ATP typical (flash)
Wavelength range	300 - 700 nm
Dynamic range	>6 decades
Fluorescence Polarization
Light source	Tungsten Halogen
Sensitivity	3 mP at 1 nM fluorescein typical
Wavelength selection	Deep blocking filters / dichroic mirrors (Top)
Wavelength range	400 - 700 nm (300 - 900 nm option)
Time-Resolved Fluorescence
Light source	High Energy Xenon Flash Lamp
Sensitivity	Europium 60 fM typical with filters (6 amol/well in 384-well plate)
Wavelength selection	Deep blocking filters / dichroic mirrors (Top) Double grating monochromator (Top / Bottom)
Wavelength range	Filters: 200 - 700 nm (900 nm option) Monochromators: 250 - 900 nm
Reagent Dispensers
Number	2 syringe pumps
Dispense volume	5 - 1000 µL in 1 µL increment
Dead volume	1.1 mL, 100 µL with back flush