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CTL Immunospot S5 Analyzer (Elispot Reader with additional functions)

/uploadedImages/MaPS/Shared_Equipment_description_pages/Picture 3.png  CTL Immunospot S5 Analyzer (Elispot Reader with additional functions)
Location: AS8-1028  Status:in service  
reserve use
Supports both visible light assays (e.g. ELISPOT, colony counting) and fluorescence-based FluoroSpot assays, as well as cell viability assays, apotosis assays, and cytotoxicity assays. Fully automated support for the following plate formats: White or transparent 96 well plates with membrane bottoms, transpararent 48 and 96 well plates with clear plastic bottoms, and standard hemocytometers.

Selectable fluorescence cubes allow ultraviolet excitation wavelengths to be fine-tuned for a wide range of fluorochromes. The standard cube provides two filters and is optimized for combinations of two fluorochromes (FITC and PE, FITC and TRITC, or other fluorochromes with similar spectral properties), with room for future expansion.

CTL ImmunoSpot® Professional Software

Unique Features include:

  • ImmunoSpot® Easy-Count: User-friendly analysis process; analyzing a plate can be as easy as one step!
  • Smart Count™: ImmunoSpot “wizard” counting mode with automated parameter set up based on proprietary counting algorithms.
  • Count Confidence™: Automatically highlights results when spot confluence, high background or the presence of artifacts, call the validity of counts in question
  • ImmunoSpot® Multi-Plate AutoCount™ (IMPAC™): Permits uninterrupted, “walk away” counting of an indefinite number of plate files.
  • ImmunoSpot® MultiSet AutoCount: Permits analysis of multiple assays within a single plate.
  • Spot Info™: Provides quantitative spot information including spot location, size, mean and maximum spot intensity, circularity, mean well intensity, plus more.
  • Autogate™: An automated “gating” algorithm that uses a scientifically validated statistical function to differentiate between signal and noise and to determine minimum and maximum spot size for different cytokines.  
  • Professional Quality Control module: Permits the verification of counts generated by automated analysis and allows for batch revisions and annotations if required to excise artifacts, to compensate for leaking membranes, etc. QC Professional mode saves time and provides consistency through the automated QC process.
  • Manual Dual Color analysis: Manual analysis of dual color spots (red, blue, and dual color) within one well.
  • Advanced presentation/publication features including direct export to PowerPoint®.