DSCL/PBCE/MBCL Equipment

    Equipment operated by the Deep Sequencing, PacBio, and Molecular Biology Cores is only accessible by Core personnel, but much of it can be utilized as a service. If the equipment you need is listed below but is not provided as an official Core Service on the appropriate page, please contact DeepSequencingCoreLabs@umassmed.edu to discuss options for sending your samples.
    Technical skills in molecular biology are also available as a service. Email MBCL@umassmed.edu to begin a discussion of your requirements.

PBCE:

Pacific Biosciences Sequel II - Single-Molecule Real-Time sequencing of long reads from 500bp to over 50kb.

DSCL:

Illumina NovaSeq 6000 - High-throughput sequencing of reads from 50bp to 150bp, rapid chemistry, ~2B reads per lane on an S2 flowcell.

Illumina MiSeq and MiSeqDx - High-throughput sequencing of reads from 25bp to 300bp, rapid chemistry, 10-20M reads per flowcell. The Dx can run clinical assays (for research use only) such as the Illumina cancer panels.

Diagenode Bioruptor Pico - Sonicator for DNA/RNA/chromatin shearing and cell or tissue disruption that uses Adaptive Cavitation. The Pico can accommodate smaller volumes than the Bioruptor NGS. This has replaced our Covaris S2.

Diagenode Bioruptor NGS - Sonicator for DNA/RNA/chromatin shearing and cell or tissue disruption that uses Adaptive Cavitation.

Covaris g-tubes - Single-use tubes for mechanical shearing of large fragments (10-30kb).

Sage Science Blue Pippin - Gel-based size-selection of fragments from 100bp to >30kb (depending on cassette and assay).

Life Technologies Qubit 3.0 Fluorometer - Fluorescence-based quantitation using intercalating dye specific for DNA, RNA, or protein (so no contamination is included). Only 1μl of sample is required.

MBCL:

ABI 3730XL Capillary Sequencer - For Sanger sequencing: the gold standard for DNA fragment analysis.

Advanced Analytical Fragment Analyzer - A capillary electrophoresis instrument for fragment analysis. This instrument has replaced the Bioanalyzer at the Core due to its higher sensitivity, wider dynamic range for assays, and throughput.

10X Genomics Chromium - For single-cell library preparation.

ABI TaqMan 7500 Fast - Real-time PCR system.

GE NanoVue Plus - A spectrophotometer capable of measuring nucleic acids, ssDNA (such as primers), protein, and fluorescent probes. Only 2-5μl of sample are required.