Antigen Retrieval Solution

Citrate Acid PH 6.0

• 1M Citrate acid stock solution:  Citrate acid monohydrate 21.01 g to 100 ml dH2O2.
• Working solution:  1:100 dilution from stock; adjust PH to 6.0.

EDTA Solution

• 0.5M EDTA (Sigma E 7889 100ml) 400 µl to 200 ml dH2O2.
• Use HCL; adjust PH to 8.0 (2M HCL add about 19 µl)