The Cardiovascular Core provides state-of-the-art phenotyping of cardiovascular system in mouse models of metabolic disease and an easily accessible cardiovascular disease model pipeline. The Core services enhance whole body metabolic characterization given that cardiovascular complications represent the major source of morbidity and mortality in obesity and diabetes. The Core also offers sophisticated disease models that encompass the three major cardiovascular complications of diabetes: peripheral vascular disease, coronary atherosclerosis, and heart failure. The Core phenotyping services include:
- 2-D and M-mode echocardiography in lightly-anesthetized mice to non-invasively assess cardiac structure and function using state-of-the-art high-frequency, high-resolution in vivo digital imaging platform (VisualSonics Vevo2100)
- Non-invasive analysis of diastolic function and strain analysis (radial, circumferential, and longitudinal) using pulsed wave color Doppler mode
- Non-invasive measurement of blood pressure, ECG, and heart rate
- In vivo assessment of endothelial function using Danish Myo Technology
- Quantification of aortic atherosclerosis via in vivo ultrasound and ex vivo en face and aortic root Oil Red O staining
- Hind Limb Ischemia (HLI) and Abdominal Aortic Aneurysm (AAA) disease models to investigate peripheral vascular disease (PVD)
- Coronary Artery Ligation (CAL) model to investigate chronic myocardial infarction
- Transverse Aortic Constriction (TAC) model to investigate pressure-overload hypertrophy, congestive heart failure, and aortic stenosis
- Left Anterior Descending Coronary Artery Ligation (LAD) Models to study both acute cardiac ischemia/reperfusion injury and chronic cardiac ischemia(MI)
- Parabiosis model surgically connecting two animals to observe secreted factor in one animals impact on the physiology of a second animal through their shared circulatory system.
John F. Keaney Jr, M.D.
University of Massachusetts Medical School