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Optical Animal Imaging Facility

INSTRUMENTS

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IVIS-100 (Perkin-Elmer)

IVIS Spectrum CT (Perkin-Elmer)

Both cameras detect bioluminescence and fluorescence contained within solutions, petri dishes, culture plates, cells or live animals.

The IVIS-100 has 4 excitation/emission fluorescent filters, covers the excitation range of 445-750 nm and emission of 500-875 nm. The chamber holds 1-5 mice or 1-3 rats in a single view. Acquisition time is about 1-3 minutes; therefore, useful for high-throughput screening. The system provides 2D imaging with photo overlay and quantification of the surface radiance of the source.

The IVIS Spectrum CT has 10 excitation and 18 emission fluorescent filters, along with spectral unmixing to facilitate the use of multiple reporters within the same subject. The system provides both 2D and 3D imaging. The 3D aspect calculates the X,Y,Z location and brightness of the source in the subject interior, and quantifies a depth corrected signal intensity. CT registration provides anatomical registration with signal at depth.

Each camera is equipped with a gaseous anesthesia vaporizer. Oxygen and isoflurane are provided.

INSTRUMENT USE

  • Any use of live animals for optical imaging requires IACUC approval. Please check that optical imaging and related procedures are included in your animal use protocol.
  • The equipment can be used without assistance after training by Core staff.
  • Core staff is available to assist with operation for studies if preferred.
  • An on-line calendar is used to reserve/cancel a time slot. The link is found at the bottom of this page, “Make a Reservation”. Be sure to sign up well in advance.
  • Each camera room has a log-in book to record arrival and departure times.

LOCATION

IVIS-100: Animal Facility, A-level, Room SA-443

IVIS Spectrum CT: Animal Facility, A-level, Room SA-414

FEES

Charges are per hour (invoiced through the Research Cores Office) and are based on time reserved in the on-line calendar and in-room record log book of arrival and departure times.

Instrument

Internal

Unassisted

Internal

Assisted

Outside Academic Assisted

External Industry Core operated

IVIS-100

$48

$173

$198

$225

IVIS Spectrum CT

$65

$200

$225

$275

Effective March 1, 2019 there is a 1 hour minimum charge for reservations. Reservations cancelled must be done 24 hours in advance.

If you are interested in using the Optical Camera, please contact:

Director, Mary Rusckowski, Ph.D.
Department of Radiology, S6-315
Mary.Rusckowski@umassmed.edu
508-856-6972

Operator,
Yuzhen Wang, Ph.D.
Department of Radiology, S6-308
Yuzhen.Wang@umassmed.edu
508-334-2296


Make a Reservation

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