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 Complete List of Published Work:

  1. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol. 2016 May;34(5):528-30. doi: 10.1038/nbt.3526. Epub 2016 Apr 18. PubMed PMID: 27088723; PubMed Central PMCID: PMC4864854. View in: PubMed.  Links: In the News
  2. Musser SM, Grunwald D. Deciphering the Structure and Function of Nuclear Pores Using Single-Molecule Fluorescence Approaches. J Mol Biol. 2016 Mar 2. pii: S0022-2836(16)00153-4. doi: 10.1016/j.jmb.2016.02.023. [Epub ahead of print] PubMed PMID: 26944195. View in: PubMed
  3. Smith C, Huisman M, Siemons M, Grunwald D, Stallinga S. Simultaneous measurement of emission color and 3D position of single molecules. Optics Express. 2016; 24:4996-5013 View article
  4. Chen J, Rahman SA, Nikolaitchik OA, Grunwald D, Sardo L, Burdick RC, Plisov S, Liang E, Tai S, Pathak VK, Hu WS. HIV-1 RNA genome dimerizes on the plasma membrane in the presence of Gag protein. Proc Natl Acad Sci U S A. 2016 Jan 12; 113(2):E201-8. View in: PubMed
  5. Smith C, Lari A, Derrer CP, Ouwehand A, Rossouw A, Huisman M, Dange T, Hopman M, Joseph A, Zenklusen D, Weis K, Grunwald D, Montpetit B. In vivo single-particle imaging of nuclear mRNA export in budding yeast demonstrates an essential role for Mex67p. J Cell Biol. 2015 Dec 21; 211(6):1121-30.
    View in: PubMed
  6. Smith C, Tu LC, Grunwald D. A 4D view on mRNA. Oncotarget. 2015 Oct 6; 6(30):28515-6.
    View in: PubMed
  7. Smith CS, Stallinga S, Lidke KA, Rieger B, Grunwald D. Probability-based particle detection that enables threshold-free and robust in vivo single-molecule tracking. Mol Biol Cell. 2015 Nov 5; 26(22):4057-62.
    View in: PubMed
  8. Smith CS, Preibisch S, Joseph A, Abrahamsson S, Rieger B, Myers E, Singer RH, Grunwald D. Nuclear accessibility of ß-actin mRNA is measured by 3D single-molecule real-time tracking. J Cell Biol. 2015 May 25; 209(4):609-19.
    View in: PubMed
  9. Chen J, Grunwald D, Sardo L, Galli A, Plisov S, Nikolaitchik OA, Chen D, Lockett S, Larson DR, Pathak VK, Hu WS. Cytoplasmic HIV-1 RNA is mainly transported by diffusion in the presence or absence of Gag protein. Proc Natl Acad Sci U S A. 2014 Dec 2; 111(48):E5205-13.
    View in: PubMed
  10. Chen J, Grunwald D, Sardo L, Galli A, Plisov S, Nikolaitchik OA, Chen D, Lockett S, Larson DR, Pathak VK, Hu WS. Cytoplasmic HIV-1 RNA is mainly transported by diffusion in the presence or absence of Gag protein. Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):E5205-13. doi: 10.1073/pnas.1413169111. Epub 2014 Nov 17. PubMed PMID: 25404326; PubMed Central PMCID: PMC4260538.  View in: PubMed
  11. Beumer KJ, Trautman JK, Christian M, Dahlem TJ, Lake CM, Hawley RS, Grunwald DJ, Voytas DF, Carroll D. Comparing zinc finger nucleases and transcription activator-like effector nucleases for gene targeting in Drosophila. G3 (Bethesda). 2013 Oct; 3(10):1717-25. View in: PubMed
  12. Nieuwenhuizen RP, Lidke KA, Bates M, Puig DL, Grünwald D, Stallinga S, Rieger B. Measuring image resolution in optical nanoscopy. Nat Methods. 2013 Jun; 10(6):557-62. View in: PubMed
  13. Singer S, Zhao R, Barsotti AM, Ouwehand A, Fazollahi M, Coutavas E, Breuhahn K, Neumann O, Longerich T, Pusterla T, Powers MA, Giles KM, Leedman PJ, Hess J, Grunwald D, Bussemaker HJ, Singer RH, Schirmacher P, Prives C. Nuclear pore component Nup98 is a potential tumor suppressor and regulates posttranscriptional expression of select p53 target genes. Mol Cell. 2012 Dec 14; 48(5):799-810. View in: PubMed
  14. Grünwald D, Singer RH. Multiscale dynamics in nucleocytoplasmic transport. Curr Opin Cell Biol. 2012 Feb; 24(1):100-6. View in: PubMed
  15. Grünwald D, Singer RH, Rout M. Nuclear export dynamics of RNA-protein complexes. Nature. 2011 Jul 21; 475(7356):333-41. View in: PubMed
  16. Lionnet T, Wu B, Grünwald D, Singer RH, Larson DR. Nuclear physics: quantitative single-cell approaches to nuclear organization and gene expression. Cold Spring Harb Symp Quant Biol. 2010; 75:113-26. View in: PubMed
  17. Dange T, Joseph A, Grünwald D. A perspective of the dynamic structure of the nucleus explored at the single-molecule level. Chromosome Res. 2011 Jan; 19(1):117-29. View in: PubMed
  18. Hocine S, Singer RH, Grünwald D. RNA processing and export. Cold Spring Harb Perspect Biol. 2010 Dec; 2(12):a000752. View in: PubMed
  19. Grünwald D, Singer RH. In vivo imaging of labelled endogenous ß-actin mRNA during nucleocytoplasmic transport. Nature. 2010 Sep 30; 467(7315):604-7. View in: PubMed
  20. Dange T, Grünwald D, Grünwald A, Peters R, Kubitscheck U. Autonomy and robustness of translocation through the nuclear pore complex: a single-molecule study. J Cell Biol. 2008 Oct 6; 183(1):77-86. View in: PubMed
  21. Grünwald D, Singer RH, Czaplinski K. Cell biology of mRNA decay. Methods Enzymol. 2008; 448:553-77. View in: PubMed
  22. Grünwald D, Shenoy SM, Burke S, Singer RH. Calibrating excitation light fluxes for quantitative light microscopy in cell biology. Nat Protoc. 2008; 3(11):1809-14. View in: PubMed
  23. Grünwald D, Martin RM, Buschmann V, Bazett-Jones DP, Leonhardt H, Kubitscheck U, Cardoso MC. Probing intranuclear environments at the single-molecule level. Biophys J. 2008 Apr 1; 94(7):2847-58. View in: PubMed
  24. Siebrasse JP, Grünwald D, Kubitscheck U. Single-molecule tracking in eukaryotic cell nuclei. Anal Bioanal Chem. 2007 Jan; 387(1):41-4. View in: PubMed
  25. Grünwald D, Spottke B, Buschmann V, Kubitscheck U. Intranuclear binding kinetics and mobility of single native U1 snRNP particles in living cells. Mol Biol Cell. 2006 Dec; 17(12):5017-27. View in: PubMed
  26. Grünwald D, Hoekstra A, Dange T, Buschmann V, Kubitscheck U. Direct observation of single protein molecules in aqueous solution. Chemphyschem. 2006 Apr 10; 7(4):812-5. View in: PubMed
  27. Grünwald D, Cardoso MC, Leonhardt H, Buschmann V. Diffusion and binding properties investigated by Fluorescence Correlation Spectroscopy (FCS). Curr Pharm Biotechnol. 2005 Oct; 6(5):381-6. View in: PubMed
  28. Kubitscheck U, Grünwald D, Hoekstra A, Rohleder D, Kues T, Siebrasse JP, Peters R. Nuclear transport of single molecules: dwell times at the nuclear pore complex. J Cell Biol. 2005 Jan 17; 168(2):233-43. View in: PubMed


  1. Dange JT, Joseph A, Grunwald D. Chromosome Research. McNally J, editor. Netherlands: Springer; 2011. Chapter 19, A perspective of the dynamic structure of the nucleus explored at the single-molecule level; p.117-129. 
  2. Grunwald D, Singer RH, Czaplinski K. Methods in Enzymology. 448 ed. Maquat L, Kiledjian M, editors. Netherlands: Elsevier Inc.; 2008. Chapter 27, Cell Biology of mRNA Decay; p.553-575.