Be careful! Only at this stage, you might damage the scope or specimen holder mechanically. Be sure that you understand what you are doing. Should you feel any confusion about this procedure, please stop and ask for help.
For the sake of filament crystal, it is REQUIRED to turn down the
filament to 10 before inserting the specimen rod. That way, in case
IGP shoots high, there will be no subtancial damage to
the LaB6 tip crystal. In general, filament should be kept at
10 or completely off until IGP
recovers to below 26.
The procedure to insert a room temperature specimen rod is below.
define airlock pumping time as 60 seconds, from Vacuum - Cryo page.
Reset stage tilt angle to 0 if it is not.
Insert rod in, with the Pin at 3'oclock position.
As soon as it reach the end, rotate rod CLOCKWISE with some pushing force so that the pin slides into the locking groove at 5'oclock position. You should feel the rod goes "in" about 8mm.
Wait until the red LED on the stage disappears. Dismiss the "non-standard" flushing message on the screen by pressing "Reset" button at lower left corner of the screen display.
Turn rod Count-Clockwise until pin is at 12'oclock position, while watching
IGP reading. You should adjust your rotating speed to keep IGP < 40.
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Electron Microscopy Facility • 55 Lake Avenue North Worcester, Massachusetts 01655-0160
Questions or Comments? Email: gregory.hendricks@umassmed.edu Phone: 508-856-2602