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Carotid Artery Ligation

Induced vascular injury models in mice have seen increased attention due to contributions of smooth muscle, endothelial, inflammatory, as well as other circulating cells leading to vascular remodeling. This remodeling creates restrictions (neointima or intimal hyperplasia) of oxygenated blood flow to critical organs and to survival.  Becoming a major cause of morbidity and mortality in cardiovascular disease patients. Performing reproducible arterial injury in the mouse model, has led the way for vascular remodeling studies, controlling vascular smooth muscle cell behavior in response to injury utilizing genetically modified mice.

Multiple Design Models For your Specific Needs

Partial carotid artery ligation involves ligating all distal branches of the left carotid artery except the superior thyroid artery, creating a low-flow state with commensurate vascular remodeling over 2 or more weeks

  • Ligation of External Carotid Artery
  • Ligation of Internal Carotid Atrery
  • Restriction of Common Carotid Artery up to 90%
  • Partial Left carotid artery ligation, ligating all distal branches except the superior thyroid artery creating a low-flow state with vascular remodeling spanned over 2 or more weeks
  • Others as requested
  • Wire Myography

Quantification of Flow:

  • VisualSonics Vivo 2100 imaging System cardiac echo
  • Micron-scale computed tomography (Micro CT/Micro PET)
  • Histological analysis

 

                                                                     Carotid Artery Vascular Remodeling Models

                                                                                                H&E (Haemotoxylin and Eosin) Staining

                           Ligation of Right External Carotid Artery                                                                              Day-3 Post Procedure 
          
      
 
 
                                  Day-7 Post Procedure                                                    Day-14 Post Procedure                                 Day-21 Post Procedure      

      

 

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