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Instrumentation

Lumos

Thermo Scientific Orbitrap Fusion Lumos Tribrid with Waters NanoAcquity UPLC

~quantitative proteomics~

A quadrupole-Orbitrap-linear ion trap tribrid mass spectrometer employing HCD, CID and ETD fragmentation options. Instrument is equiped with a FAIMS PRO source for atmospheric ion mobility separations providing improved analyte selectivity and expanded proteome coverage. Analysis of complex protein digests including identification of post-translational modifications, covalently bound protein-drug adducts, and chemically cross-linked peptides. Ideal for stable isotope label (e.g. SILAC, TMT) or label-free (peptide peak area) quantitative proteomics workflows.

   

Thermo Scientific Q Exactive with Waters NanoAcquity UPLC

~quantitative proteomics~

A quadrupole-Orbitrap hybrid mass spectrometer employing HCD fragmentation used for quantitative proteomics applications. Analysis of simple and complex protein digests including identification of post-translational modifications, covalently bound protein-drug adducts, and chemically cross-linked peptides. Ideal for stable isotope label (e.g. SILAC, TMT) or label-free (peptide peak area) quantitative workflows.

 

Thermo Scientific Q Exactive HF-X with Thermo Vanquish Flex UHPLC

~metabolomics profiling and targeted quantitation~

A quadrupole-Orbitrap hybrid mass spectrometer employing HCD fragmentation and conventional flow UHPLC. Metabolomics applications include untargeted metabolomic and lipidomic profiling and targeted assays.

 

Waters Synapt G2-Si

A quadrupole time-of-flight mass spectrometer enabled with gas-phase ion mobility separations for improved analyte selectivity.

~mass spectrometry tissue imaging~

  • in-situ tissue imaging for mapping the spatial distribution of exogenous drugs or endogenous lipids and metabolites in sectioned tissue.
  • Nd:YAG high repetition laser for matrix-assisted laserdesorption/ionization (MALDI) applications.
  • HTX Technologies TM-Sprayer for matrix application.
  • desorption electrospray ionization (DESI) option available.

~intact protein, mAb, and oligonucleotide analysis~

  • intact and reduced proteins, mAb's, and oligonucleotides analyzed by ESI LC-MS with a Waters Acquity UPLC. UPLC fitted with an inline UV detector.
  • nanoflow UPLC options also available.
 

Waters Xevo TQ-XS with Waters Aquity UPLC

 ~targeted metabolomics~

A triple quadrupole mass spectrometer for targeted metabolomics and small molecule quantitation using conventional flow UPLC. UPLC fitted with an inline photodiode array detector.

 

Thermo Scientific TSQ Quantiva with Dionex UltiMate 3000 UHPLC

 ~targeted metabolomics~

A triple quadrupole mass spectrometer for targeted metabolomics and small molecule quantitation using conventional flow UHPLC. A Proxeon EasyLC is also available for nanoflow applications.

 

~Legacy Instruments~

 

Thermo Scientific Orbitrap Velos Pro with Thermo Accela 1250 UHPLC or Waters NanoAcquity UPLC

A linear ion trap-Orbitrap hybrid mass spectrometer employing CID, HCD, and ETD fragmentation. Untargeted metabolomic and lipidomic profiling and targeted assays using HCD fragmentation using conventional flow UHPLC. Nanoflow UPLC for high-sensitivity applications including neuroactive steroids, modified nucleotides, and peptides.

 

Thermo Finnigan LTQ with Thermo Accela 1250 UHPLC

A linear ion trap mass spectrometer with conventional flow UHPLC for nominal mass measurement of small molecules and general LC-MS method development.

 

Shimadzu Axima TOF2

A matrix-assited laser derpsortion/ionization tandem time-of-flight (MALDI TOF-TOF) instrument for the analysis of low molecular weight proteins, peptides, polymers, carbohydrates, and lipids. High energy CID, linear and reflectron acquisition modes, in-source and post-source decay options.

The Mass Spectrometry Facility
University of Massachusetts Medical School

Fuller Building
222 Maple Avenue
Shrewsbury, MA 01545
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