Proteomics and Mass Spectrometry Facility
UMass Medical School

Fuller Building
222 Maple Avenue
Shrewsbury, MA 01545


     •Quantitative Proteomics
     •Small Molecule Quantitation
     •Separations Science

Much of what we do can be broadly grouped accordingly.  Below is a list of technology approaches and application areas.  If you don't find what you are looking for, please inquire within.

  1. Electrospray ionization (ESI) liquid chromatography tandem mass spectrometry (LC-MS/MS)

    Comprehensive peptide identification of simple and complex protein digests from gel bands (e.g. Coomassie blue and silver stained), immunoprecipitation (IP), and whole-cell/secreted/tissue digests by shotgun proteomics. Approaches for maximizing proteomic coverage, peptide quantitation, and de novo peptide sequencing offered.  Identification and localization of post-translational modifications, chemically cross linked peptides, protein-drug adducts and disulfide mapping.  Whole protein analysis is also available.  LC-MS profiling, exact mass measurement, structure elucidation and quantitation of small molecules (drugs, contaminants), lipids, glycolipids, and metabolites.  Applications to drug metabolism, pharmacokinetics, lipidomics, and metabolomics.
  2. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry

    Peptide identification of simple protein digests from gel bands/2D spots (e.g. Coomassie blue and silver stained) and purified proteins.  De novo peptide sequencing and In-Source-Decay (ISD) N-terminal/C-terminal sequence verification of purified recombinant proteins.  Identification and localization of chemical and post-translational modifications.  Analysis of whole proteins, synthetic polymers, carbohydrates, and glycolipids.
  3. Protein and peptide fractionation

    Fractionation technologies include one-dimensional gel electrophoresis, immuno-depletion of abundant proteins from biological fluids, and semi-preparative liquid chromatography (e.g. strong cation exchange, reverse-phase, normal phase).
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