Page Menu

Pacific Biosciences Core Enterprise

PBCE logo

    The Core operates a Pacific Biosciences Sequel II Instrument with the newest version of the run chemistry (read lengths over 50kb) and SMRTlink data analysis. The service includes (as a package deal): library construction from submitted DNA or RNA, sequencing of one SMRTcell, and quality filtering/run metrics. Multiplexing is available using either internal PacBio Barcoded Universal Primers (for amplicon libraries) or PacBio Barcoded Adapters. Additional bioinformatics options are available for a fee. Please contact us about your project. We'd love to work with you!



  • Our package deal includes library building and sequencing of one SMRTcell.
  • Additional SMRTcells can be added at a discounted price.
  • Multiplexed library builds are available. The options for this are: barcodes internal to your amplicon(s) (you would have to add these during amplification using PacBio's Barcoded Universal Primers) or barcodes in the PacBio SMRTbell adapters (added by the Core during library prep).
  • Bioinformatics services offered include consensus (ROI) analysis, IsoSeq analysis, variant, motif, and/or base modification detection, assembly, and mapping options.
  • Material may be turned in as RNA or DNA.SMRTbell Template
Library Types:
Amplicon or small fragment
IsoSeq (cDNA)
Genomic or plasmid DNA
Indexed (for multiple lib. types)
Imaging Times:
10 hours
(standard run)
20 hours
(for very large inserts; extra cost)


Drop-off locations:
Samples can be left in the labeled fridges at these locations
for 11:00 AM pickup each business day:
Biotech2 outside Suite 207
LRB 6th floor mailroom
ASC8-2058 mailroom
We are also available by appointment if you need to visit with us.
Our shipping address for off-campus customers is
Rose-Gordon Bldg. Rm. 141, 222 Maple Ave, Shrewsbury, MA, 01545.

    Data delivery options for non-UMass customers are to have the data uploaded to an outside server (using SFTP) or transferred to an external drive meeting our security/speed requirements and shipped overnight.


The PacBio Core Enterprise would be pleased to have you mention us in the acknowledgements section of any publications or presentations of data generated with our support.
If you would like to have your publication listed on the Core's website to help highlight the work UMass researchers are doing, email us the reference at

A collection of papers highlighting applications possible with PacBio technology

RADAR-seq: A RAre DAmage and Repair sequencing method for detecting DNA damage on a genome-wide scale

The Origin of the Haitian Cholera Outbreak Strain

Direct Detection and Sequencing of Damaged DNA Bases

Direct Detection of DNA Methylation During Single-Molecule, Real-Time Sequencing

Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing

Validation of ITD mutations in FLT3 as a therapeutic target in human acute myeloid leukaemia

Going beyond five bases in DNA Sequencing

Additional Resources
click to open search panel