Title: my5C Software. UMMS09-57; Patent Pending.
My5c software is a publicly available computational tool that can be used for 5C-based chromosome structural studies. 5C is a powerful high-throughput method for analyzing chromosome folding. Researchers can upload data obtained from their experiments, either by sequencing or microarray. Using My5C, researchers can obtain a two-dimensional heat map of three-dimensional chromosome structure.
This invention provides a new protocol to describe long range physical interaction of genome loci within and between chromosomes. Long range regulatory elements in the genomes are known to interact. This interaction is important for genome biology, as it can control aspects of the genome, such as stability and dosage compensation. Contrary to the previous methods for describing long range genome interactions, Hi-C does not require prior understanding of one genomic loci, making it a truly genome wide characterization. This invention may reveal important aspects of genomic interaction in disease.