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Thyme Lab Publications

All Thyme Lab Publications on PubMed

Total: displaying 10 out of 29 results
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  • Genetic context of transgene insertion can influence neurodevelopment in zebrafish

    lunes, marzo 17, 2025
    Author(s): Anna J Moyer,Alexia Barcus,Mary E S Capps,Jessica A Chrabasz,Robert L Lalonde,Christian Mosimann,Summer B Thyme
    Source: bioRxiv : the preprint server for biology
    The Gal4/ UAS system is used across model organisms to overexpress target genes in precise cell types and relies on generating transgenic Gal4 driver lines. In zebrafish, the Tg(elavl3:KalTA4) ( HuC ) Gal4 line drives robust expression in neurons. We observed an increased prevalence of swim bladder defects in Tg(elavl3:KalTA4) zebrafish larvae compared to wildtype siblings, which prompted us to investigate whether transgenic larvae display additional neurobehavioral phenotypes. Tg(elavl3:KalTA4)...

  • Impairments of cerebellar structure and function in a zebrafish KO of neuropsychiatric risk gene znf536

    jueves, febrero 08, 2024
    Author(s): Tae-Yoon Kim,Arkaprava Roychaudhury,Hyun-Taek Kim,Tae-Ik Choi,Seung Tae Baek,Summer B Thyme,Cheol-Hee Kim
    Source: Translational psychiatry
    Genetic variants in ZNF536 contribute to the risk for neuropsychiatric disorders such as schizophrenia, autism, and others. The role of this putative transcriptional repressor in brain development and function is, however, largely unknown. We generated znf536 knockout (KO) zebrafish and studied their behavior, brain anatomy, and brain function. Larval KO zebrafish showed a reduced ability to compete for food, resulting in decreased total body length and size. This phenotype can be rescued by...

  • Mitochondrial prodha regulates proliferation of radial glia-like cells in the zebrafish hindbrain

    martes, diciembre 19, 2023
    Author(s): Philip D Campbell,Isaiah Lee,Summer Thyme,Michael Granato
    Source: Molecular psychiatry
    No abstract

  • Mitochondrial proteins encoded by the 22q11.2 neurodevelopmental locus regulate neural stem and progenitor cell proliferation

    miércoles, octubre 04, 2023
    Author(s): Philip D Campbell,Isaiah Lee,Summer Thyme,Michael Granato
    Source: Molecular psychiatry
    Microdeletion of a 3Mb region encompassing 45 protein-coding genes at chromosome 22q11.2 (22q11.2DS) predisposes individuals to multiple neurodevelopmental disorders and is one of the greatest genetic risk factors for schizophrenia. Defective mitochondrial function has been hypothesized to contribute to 22q11.2DS pathogenesis; however, which of the six mitochondrial genes contribute to neurodevelopmental phenotypes and their underlying mechanisms remain unresolved. To systematically test...

  • Behavioral analysis through the lifespan of disc1 mutant zebrafish identifies defects in sensorimotor transformation

    viernes, julio 07, 2023
    Author(s): Brock R Pluimer,Devin L Harrison,Chanon Boonyavairoje,Eric P Prinssen,Mark Rogers-Evans,Randall T Peterson,Summer B Thyme,Anjali K Nath
    Source: iScience
    DISC1 is a genetic risk factor for multiple psychiatric disorders. Compared to the dozens of murine Disc1 models, there is a paucity of zebrafish disc1 models-an organism amenable to high-throughput experimentation. We conducted the longitudinal neurobehavioral analysis of disc1 mutant zebrafish across key stages of life. During early developmental stages, disc1 mutants exhibited abrogated behavioral responses to sensory stimuli across multiple testing platforms. Moreover, during exposure to an...

  • Multiple adaptations underly co-option of a CRISPR surveillance complex for RNA-guided DNA transposition

    viernes, junio 02, 2023
    Author(s): Jung-Un Park,Michael T Petassi,Shan-Chi Hsieh,Eshan Mehrotra,Gabriel Schuler,Jagat Budhathoki,Vinh H Truong,Summer B Thyme,Ailong Ke,Elizabeth H Kellogg,Joseph E Peters
    Source: Molecular cell
    CRISPR-associated transposons (CASTs) are natural RNA-directed transposition systems. We demonstrate that transposon protein TniQ plays a central role in promoting R-loop formation by RNA-guided DNA-targeting modules. TniQ residues, proximal to CRISPR RNA (crRNA), are required for recognizing different crRNA categories, revealing an unappreciated role of TniQ to direct transposition into different classes of crRNA targets. To investigate adaptations allowing CAST elements to utilize attachment...

  • A single-cell transcriptome atlas of the maturing zebrafish telencephalon

    martes, abril 18, 2023
    Author(s): Shristi Pandey,Anna J Moyer,Summer B Thyme
    Source: Genome research
    The zebrafish telencephalon is composed of highly specialized subregions that regulate complex behaviors such as learning, memory, and social interactions. The transcriptional signatures of the neuronal cell types in the telencephalon and the timeline of their emergence from larva to adult remain largely undescribed. Using an integrated analysis of single-cell transcriptomes of approximately 64,000 cells obtained from 6-day-postfertilization (dpf), 15-dpf, and adult telencephalon, we delineated...

  • Mitochondrial genes in the 22q11.2 deleted region regulate neural stem and progenitor cell proliferation

    lunes, enero 30, 2023
    Author(s): Philip D Campbell,Isaiah Lee,Summer Thyme,Michael Granato
    Source: bioRxiv : the preprint server for biology
    Microdeletion of a 3Mbp region encompassing 45 protein-coding genes at chromosome 22q11.2 (22q11.2DS) predisposes to multiple neurodevelopmental disorders and is one of the greatest genetic risk factors for schizophrenia. Defective mitochondrial function has been hypothesized to contribute to 22q11.2DS pathogenesis; however, which of the six mitochondrial genes contribute to neurodevelopmental phenotypes and their underlying mechanisms remain unresolved. To systematically test 22q11.2DS genes...

  • Mosaic and non-mosaic protocadherin 19 mutation leads to neuronal hyperexcitability in zebrafish

    sábado, abril 23, 2022
    Author(s): Barbara K Robens,Xinzhu Yang,Christopher M McGraw,Laura H Turner,Carsten Robens,Summer Thyme,Alexander Rotenberg,Annapurna Poduri
    Source: Neurobiology of disease
    Epilepsy is one of the most common neurological disorders. The X-linked gene PCDH19 is associated with sporadic and familial epilepsy in humans, typically with early-onset clustering seizures and intellectual disability in females but not in so-called 'carrier' males, suggesting that mosaic PCDH19 expression is required to produce epilepsy. To characterize the role of loss of PCDH19 function in epilepsy, we generated zebrafish with truncating pcdh19 variants. Evaluating zebrafish larvae for...

  • ZebraShare: a new venue for rapid dissemination of zebrafish mutant data

    jueves, mayo 06, 2021
    Author(s): April DeLaurier,Douglas G Howe,Leyla Ruzicka,Adam N Carte,Lacie Mishoe Hernandez,Kali J Wiggins,Mika M Gallati,Kayce Vanpelt,Frances Loyo Rosado,Katlin G Pugh,Chasey J Shabdue,Khadijah Jihad,Summer B Thyme,Jared C Talbot
    Source: PeerJ
    CONCLUSION: ZebraShare provides an opportunity for researchers to directly share information about mutant lines within ZFIN, which is widely used by the community as a central database of information about zebrafish lines. Submissions of alleles with a phenotypic or unexpected phenotypes is encouraged to promote collaborations, disseminate lines, reduce redundancy of effort and to promote efficient use of time and resources. We anticipate that as submissions to ZebraShare increase, they will...
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