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Melissa J Moore

 

 

 

 

UMassMed Faculty Page

Inventions:

Title: Developing of Soluble FLT1 Targeting Therapeutics for Treatment of Preeclampsia. UMMS15-50. Patent Pending.

  • Selective elimination (filtration) of circulation "soluble fms-like tyrosine kinase 1" proteins (sFLT1s) in women with preeclampsia is a successful strategy for treatment of preeclampsia. The invention comprises oligonucleotide compounds that bind intronic regions of mRNA encoding sFLT1 protein, thereby inhibiting expression of sFLT1 protein for treatment of preeclampsia and other angiogenic disorders. Targeting these regions with RNAi compounds enables selective silencing of truncated, soluble variants without interfering with regular FLT1 function. Further, there is no detectable transfer of the oligonucleotides to the fetus. 

Title: Nucleic Acid-guided, Thermostable Restriction Enzyme for DNA and RNA. UMMS15-41.

  • Our invention is a method for the creation of a thermostable, programmable restriction enzyme that can be used to cleave ssRNA, ssDNA, dsRNA, or dsDNA at a specific site determined by the sequence of the small DNA guide bound to Thermus thermophilus Argonaute (TtAgo) protein. This invention is a method that can be used as a molecular biology research tool for cloning and/ manipulating DNA or RNA for research use. 

Title: OmniPrep: An Optimized Method for Making Strand-specific Deep Sequencing Libraries from Diverse RNA Inputs and Small Sample Sizes. UMMS14-03; Patent Pending.

  • This invention provides novel methods for generating cDNA libraries that allow for reliable mapping of the 5′ and 3′ ends of transcripts as well as mapping, to a single nucleotide, the length of the poly(A) tail. This technology overcomes the limitations and biases present in current deep-sequencing platforms. 

Title: Genome-wide Mapping of 5' and 3' End Sequences as well as Poly(A) Tail Length of Capped Transcripts with Single-nucleotide Resolution Using High-throughput Sequencing. UMMS13-35; Patent Pending. 

  • This invention provides novel methods for generating cDNA libraries that allow for reliable mapping of the 5′ and 3′ ends of transcripts as well as mapping, to a single nucleotide, the length of the poly(A) tail. This technology overcomes the limitations and biases present in current deep-sequencing platforms.

Title: DEDUCING EXON CONNECTIVITY BY RNA-TEMPLATED DNA. UMMS09-38; Patent 8,975,019

  • This invention discloses a method for studying mRNA sequences without disrupting transcript connectivity or relative abundance. Contrary to existing methods to study mRNA splicing, this method directly assesses the relative abundance of isoforms, with information regarding multiple ligases and mRNA exon connectivity. Improved understanding of isoforms may facilitate further study of developmental disease or cancer.

Title: Mammalian Cell Lines for High Throughput Screening for mRNA Splicing Inhibitors. UMMS09-06.

  • This invention contains a method for high throughput screening for drug compounds that inhibit mRNA splicing. Using a luciferase based assay system, the inventors developed an assay that can report when splicing has occurred. Drugs that can inhibit major and minor splicesomes can be identified through this method. This technique is applicable to diseases that arise from perturbations in mRNA processing and/or splicing. 

 

Innovation TopicsRNAi/micro-RNA TherapyEclampsia/PreeclampsiaObstetricsResearch ToolsRestriction enzymeSequencing, GenomicsTranscriptomicsGene expressionAutismAmyolateral Sclerosis (ALS)