The UMMS RNAi Core Facility houses the complete collections of human and mouse retroviral and lentiviral short hairpin RNA (shRNA) libraries from Open Biosystems, and the Mammalian Gene Collection (MGC) cDNA Library. They are available for distribution to UMass researchers (Amherst, Boston, Dartmouth, Lowell and Worcester campuses ONLY) at a reduced price.
We currently have the following genome-wide libraries available for distribution:
Complete information regarding the retroviral and lentiviral shRNAmir libraries and thelentiviral TRC libraries can be found on the Open Biosystems website. In addition, please refer to the Nature Methods paper and Nature Genetics Technical Report for information on the libraries.
shRNAs can be purchased as either single clones, pools (consisting of 5000 shRNAs/pool) for genome-wide screening, or sub-libraries in pool format.
The Mammalian Gene Collection (MGC) cDNA Library contains a total of 16,953 human (6609 clones; 5537 unique sequences) and mouse (10,344 clones; 7718 unique sequences) sequence-validated full-length cDNA sequences in an expression vector (pCMV-Sport6). Information about the MGC library and the pCMV-Sport6 expression vector can be found at:http://mgc.nci.nih.gov/ and http://mgc.nci.nih.gov/Vectors/vec_pcmv-sport6. [NOTE: These websites are for informational purposes only. Do NOT use the MGC website to look up clone ID numbers; instead, download the list of available clones from our website (see below)].
The libraries are available to distribute to UMass researchers (Amherst, Boston, Dartmouth, Lowell and Worcester campuses) ONLY. Individual clones are available in DNA format (10ug) at the cost of $50 each. We also have plasmid preparation service at a cost of $20 for >200ug.
If you would like to check the availability of your clone, please log on to the order form and download the list of cDNA clones (in PDF format) we have in the core facility.