Proteomics and Mass Spectromy Facility
UMass Medical School

Fuller Building
222 Maple Avenue
Shrewsbury, MA 01545
Inquiries

Request for Services

Information for Project Initiation, Sample Submission, and Inquiries

To initiate a project, submit a sample, or schedule ¬≠¬≠instrument time, please follow the WORKFLOW described (on right).

All projects and services are subject to approval by the Facility Director.  Most new projects require a consultation prior to initiation of work.  Consultations include advice on the best experimental approach, timing of the experiment, sample preparation and charges.  Samples are accepted only after the project has been approved and assigned to a Facility staff member.

Rates.  Our rate sheet captures the per sample cost or hourly rate for most of our services.  In general, costs for most proteomics projects can be determined from the rate sheet.  However, most small molecule projects are quotated by proposal and initiate following mutual acceptance. 

The Facility will not accept BIOHAZARDOUS and RADIOLABELED samples.

RG-3 or select agents (http://www.selectagents.gov) WILL NOT BE ACCEPTED unless inactivated by a process approved by an Institutional Biosafety Committee (IBC).

Guidelines for submitting samples for protein analysis

Excised gel bands should be placed in 0.5 ml microcentrifuge tubes previously washed with 1% formic acid in 50% ethanol for 5 minutes (discard wash).

****Keratin contamination will always be observed.
Precautions must be taken to minimize contamination****
1. Wear gloves at all times and work with a mask or shield when cutting gel bands.
2. Thoroughly wash and methanol rinse anything that will come in contact with the sample, i.e., gel apparatus, staining trays, gel excising equipment and tubes.  Rinse the microcentrifuge tube and its cap with 1% formic acid in 50% ethanol before putting in the gel band.

 

Guidelines for shipping

In general, most samples should be shipped in a sealed container containing dry ice. For compliance on packaging and transport of hazardous substances see IATA (http://www.iata.org/whatwedo/cargo/dangerous_goods/Pages/infectious_substances.aspx) and DOT (http://www.phmsa.dot.gov/hazmat) for additional guidance.

Samples may be shipped by overnight service to:

Dr. Hien Nguyen
University of Massachusetts Medical School 
Proteomics and Mass Spectrometry Facility
Fuller Building 
222 Maple Avenue 
Shrewsbury, MA 01545

WORKFLOW

  1. Initiate a project by emailing proteomics@umassmed.edu  Provide a brief description of your project request.  If you are a student or postdoc, please indicate the PI's laboratory to which you belong. 
  2. The Director or one of the laboratory staff will contact you to schedule a consultation and to confirm approval of your project.
  3. Consultation will be in person, via email or by phone.
  4. Upon project acceptance and assignment to a staff member, future consultation and sample submission for your project will continue via the assigned staff member. 
  5. New projects must be initiated by following Step 1 (above).