Proteomics and Mass Spectromy Facility
UMass Medical School

Fuller Building
222 Maple Avenue
Shrewsbury, MA 01545
Inquiries

Instrumentation

Instramentation

Thermo Scientific LTQ Orbitrap Velos Pro with Proxeon EasyLC

A linear ion trap-Orbitrap hybrid mass spectrometer employing CID, HCD, and ETD fragmentation techniques for analysis of complex protein digests or top-down whole protein analysis. Identification of post-translational modifications including phosphorylation, acetylation, methylation, ubiquitination and chemical modifications including protein-drug adducts and chemically cross-linked peptides. Ideal for stable isotope label (e.g. SILAC) or label-free quantitative proteomics approaches.

 
Instramentation Thermo Scientific Q-Exactive with Waters NanoAcquity UPLC or Thermo Accela 1250 UPLC

A quadrupole-Orbitrap hybrid mass spectrometer employing HCD fragmentation for the analysis of complex protein digests, small molecule profiling/quantitation, or whole protein analysis. Identification of post-translational modifications and chemically cross-linked peptides. Ideal for stable isotope label (e.g. SILAC, TMT, iTRAQ) or label-free quantitative proteomics approaches. Nanoflow (NanoAcquity) and conventional flow (Accela) UPLC allow for a wide range of chromatographic applications.

 
Instramentation Agilent Technologies 6520 Q-TOF with Infinity 1260 HPLC

A quadrupole time-of-flight mass spectrometer for metabolomic and small molecule quantitative applications.

 
Instramentation Waters Quattro Premier XE triple quadrupole with Waters Acquity UPLC or Waters NanoAquity UPLC

A triple quadrupole mass spectrometer for quantitation of small molecules and peptides. Conventional flow (Acquity) and nanoflow (NanoAcquity) UPLC allow for a wide range of chromatographic applications.

 
Instramentation

Shimadzu Axima TOF2 (TOF-TOF)

A MALDI time-of-flight tandem instrument for high energy CID analysis of whole proteins, peptides, polymers, carbohydrates, and glycolipids. Top-down protein sequencing by in-source decay, peptide de novo sequencing by post-source decay.

 
Instramentation

Waters Q-TOF Premier with Advion Triversa Nanomate or HDX MS interface

A quadrupole time-of-flight hybrid mass spectrometer for autmated (Nanomate) exact mass measurment of small molecules or whole proteins. Hydrogen-deuterium (H/D) exchange protein confirmation studies using an online pepsin HDX interface coupled to a CID MSE data-acquisition platform.

 
Instramentation

Thermo Scientific LTQ with Advion Triversa NanoMate or Thermo Surveyor HPLC

A linear ion trap mass spectrometer for the analysis of small molecules introducted by automated infusion (Nanomate) or HPLC (Surveyor).