Peptide Core Facility

The Peptide Core Facility, located in Room S4-714 in the Physiology Department, provides

Peptide synthesis is performed by solid-phase methodology (Fmoc amino acids and HBTU activation) using an advanced Apex 396 fully automated synthesizer with robotic features that ensure precise liquid handling and permit the synthesis of 6 to 96 peptides concurrently.

Fmoc solid-phase peptide synthesis is the most reliable and widely used method in the industry. Our peptides are de-salted by Sep-Pak extraction or subjected to HPLC and then lyophilized to obtain the product as a white powder.

In general, our peptide services include peptides in desired quantities discounted by scale (25µmol, 50µmol, or 100µmol) and purification available from 70% to over 95% purity.

 

 Peptide Core Facility 

Peptide Core Facility 

HPLC Chromatography

Peptide Core Facility 

For synthetic peptides, reverse-phase HPLC is performed on the entire batch of material obtained from the synthesizer instrument. Preparative scale (up to 200 mg) reverse-phase HPLC is routinely performed using acetonitrile gradients and Water's equipment with variable wavelength spectrophotometric detection.

Sep-Pak Purification

For 70% purity, the peptide can be Sep-Pak purified and then lyophilized.

Amino Acid Analysis

Amino acid analysis involves pre-column derivitization with Accu-Tag reagent, followed by automated HPLC separation and computerized peak integration using the Water's Millenium system.

Peptide Conjugation

Peptides are routinely conjugated to proteins to prepare immunogens with incorporations of up to 1000 mol/mol.  Generally, this involves the use of enriched forms of KLH or BSA and coupling reagents that specifically crosslink Lys or Tyr residues, followed by dialysis and lyophilization.

Sequence Consultation

We offer free advice to optimize the design of your peptide to accomplish your goals and to avoid problematic residues