Below are posted questions and answers about the EconoSEQ program services.
We are updating this constantly, thank you for your patience.
Do you pick up samples or must we bring them to you ?
At this time we do not have resources for a pick up service.
We may add this in the future if there is sufficient interest.
Where are you located ?
The drop off location is in 2 Biotech. There is a fridge across
from Lab 207. Please leave samples in the fridge and sign the
log book so that we know what order samples arrived.
Your sample ticket should be emailed to MBCL@umassmed.edu
When should I get my samples there and how long will they take ?
Samples turned in by 2:30 pm are processed overnight and placed
on the analyzer the following morning. If there aren't many samples
you may get your data that afternoon, if there are several racks of
samples being processed it might be the next morning.
How do I get my data and what form will it be ?
The default delivery option is mounting the data to the PMM server.
When your samples have been processed, you will receive an
email with download instructions. If you wish to have your data
mounted on a flash drive, please drop it off with your samples. If
you have your own server or special delivery issues, please let us know.
The data is generally delivered as .ab1 files which can be read by most
trace viewers (free ones are listed on the resources page in the left nav).
Why do I have to put my samples in a 96 well PCR plate or those strip tubes ?
The EconoSEQ option is a way for us to work small numbers of individual
samples into the HTP (High Through-Put) sequencing operation. We combine smaller
numbers of samples into single 96 well blocks for processing. Samples which are
compatible with the handling system can easily be processed. If you need
strip tubes or PCR plates, we sell them here in small packages for $10. Even
if you only have 1 sample, it's OK to put it in a 96 well plate (just mark the
plate so we can find it). Samples submitted in larger tubes or with templates and
primers in separate tubes must wait until someone has time to set them up.
What type of instrument and reagents are you using ?
We use Applied Biosystems BigDye V3.1 Dye terminators. The instrument used
is an ABI3730xl 96 capillary DNA analyzer, named Rosalind.
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