Section: Rotations

Madelyn Schmidt, Ph.D.

Academic Role: Assistant Professor

Faculty Appointment(s) In:
   Molecular Genetics and Microbiology
   Physiology

Other Affiliation(s):
   Program in Immunology and Virology

Potential Rotation Projects

Project #1. Gene therapy for prostate cancer. The rotation is to produce and test adenovirus vectors that express various forms of FasL and an antisense for the metalloproteinase, MMP-7. The student will learn about cloning, the use of fluorescent reporters, the preparation and use of adenovirus stocks, and analysis of gene expression and cell populations using the fluorescence activated cell sorter and Western blotting. In vivo experiments in mice will also be carried out.

Project #2. Analysis of mechanism of a persistent RNA virus (VSV) infection of primary lymphocytes. The rotation will involve an in vitro analysis of virus persistence in primary B cells. The student will learn preparation of primary cells, analysis of virus RNA, protein and virion production, and construct and use AdV expressing different inhibitors or activators of signaling pathways to investigate the mechanism of antibody inhibition.

Office: S5-242
Phone: 508-856-6040
E-mail: Madelyn.Schmidt@umassmed.edu
Keywords: Immunology, Cancer Biology, Virology

More on Madelyn Schmidt's Research Research | Publications | Rotations View All Sections on One Page