Transgenic Animal Modeling Core

Stephen Jones, PhD
Director

 

Services 
Fees 
Contact Us 

Day 3.5 mouse blastocyts

Purpose 

The Transgenic Animal Modeling Core assists DERC investigators with mouse experiments.  We consult with investigators to create genetically modified mice in a timely and efficient manner.

Services 

  • Transgenic mice and rats
  • Lentiviral injections into mice or rats
  • Gene targeting in mouse ES cells
  • Production of Chimeric mice using ES cells
  • Sperm cryopreservation with in vitro fertilization
  • Rederivation of mice to obtain SPF status
  • Backcrossing of mice with specified strains

For a more complete description of these services, please visit the Transgenic Animal Modeling Core website.

Fees

(DERC members have priority in obtaining core services.   Note that acknowledgement of the use of DERC Scientific Core resources is required in any resultant publication, paper, or a presentation.)

DERC Members

Other UMMS

Transgenic Services (per construct)
   Pronuclear injection-C57B1/6 inbred

 $4560

 $5700

   Pronuclear injection-FVB inbred

 $3648

 $4000

   Pronuclear injection-B6XSJL

 $2800

 $3500

   Specified inbred strain

 Please inquire

   Lentiviral injection into
   C57b1/6 mouse oocytes

 $1920

 $2400

   Pronuclear injection-SD (rat)

 $1600

 $2000 + cost of rodents

   Lentiviral injection into fertilized rat or
   mouse oocytes

 $1600

 $2000 + cost of rodents

Gene Targeting (per construct)

   
   ES cell clone isolation-per electroporation

 $2000

 $2500

   Additional Cre excision

 $320

 $400

   Allele jiumping in ES cells

 $400

 $500

Chimeric Mice Production (per ES Clone)    
   Blastocyst Injection-C57B1/6 inbred

 $3648

 $4000

Cryopreservation Services (per line)    
   Embryo vetrification

 $1288

 $1610

   Regeneration of cryopreserved line

 $240

 $300

   Sperm cryopreservation

 $240

 $300

Other Services    
   Strain rederivation (embryo transfer-perline)

 $800

 $1000

   Breeding of mice for germline  transmission
   (per mouse)

 $120

 $150

   Backcross-standard (per generation)

 $120

 $150

   Backcross-embryo transfer (per generation)

 $800

 $1000

   In vitro fertilization (hybrid strain)

 $1000

 $1250

Contact Us

Stephen Jones, PhD, Director
Phone: 508-856-7500
Email:  stephen.jones@umassmed.edu 

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