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Purpose
The Cell Science Core facilitates cell culture applications for DERC investigators by offering discounted tissue culture media products and serum along with microbiological media preparation services. Tissue culture media can be either conveniently ordered for next day delivery or picked up for immediate use (commonly used media products only).
The UMASS Tissue Culture Facility and Media Center serves as the DERC Cell Science Core and welcomes all UMass investigators as well as outside investigators.
Services
Tissue and cell culture
- Monoclonal antibody production and purification
- Hybridoma production (in collaboration with investigator)
- Cell cloning (hybridoma and transfected cells)
- Routine maintenance cell lines in monolayer and suspension culture
- Cell production (monolayer, suspension and spinner culture)
- Preparation of specialized media and buffers
- Programmable cryopreservation of tissue or cell stocks for higher viability
- Preparation and freezing of cell stocks
- Centralized cell storage in liquid nitrogen
- Primary culture (e.g. from skin biopsy, vascular endothelium, or other tissues, done in collaboration with investigator)
- Mycoplasma testing of cell lines
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