Immuno-Electron Microscopy

Immuno-electron microscopy is used to localize molecules at the ultrastructural level by labeling them with specific antibodies. The antibodies are visualized by electron-opaque markers (colloidal gold particles) attached to them. The effect is to produce an electron-dense label at the site of the antigen-antibody reaction. When the antigen in question is located on the surface of the specimen, these techniques can be used in conjunction with scanning electron microscopy. The electron dense label can then be viewed using the back scatter image on an appropriately equipped microscope.

Double labeled cell membrane fragments

Double labeled cell membrane fragments. large gold beads- glucose transporter, Glut4; small gold beads, vimentin. (Courtesy of Drs Michael Czech and Adilson Guilherme, Biochemistry Molecular Biology, UMass Medical School)