Negative Staining

Negative staining is used to visualize the detailed structure of isolated organelles, viruses, macromolecular assemblies and single protein molecules. The technique is very simple and involves surrounding the particles with an amorphous layer of heavy metal salts. The stain penetrates into the interstices of the particles to bring out their fine structural detail in negative contrast.

Actin filaments

Negatively stained actin filaments. Photo: Craig Lab.

Acto S1

Negatively stained actomyosin filaments. Photo: Craig Lab.