Core Facilities

Electron Microscopy Core Facility

EM scopeDirected by Roger Craig, PhD, and George Witman, PhD, and managed by Greg Hendricks, PhD. The facility is equipped with one scanning and three transmission electron microscopes together with the ancillary equipment required to carry out all key ultrastructural procedures from the tissue to the molecular level. The facility provides full service in specimen preparation, microscopy and interpretation of results, all at very reasonable rates. Training and use of all the equipment are also available and encouraged.

Transgenic and Knock-out Mouse Core Facility

Mouse CoreDirected by Stephen Jones. Gene Targeting component is supervised by Marilyn Keehler. Animal knock-out component is managed by Joe Gosselin. The mission of the Transgenic Animal Modeling Core is to produce genetically-altered mice for the UMASS Medical School Community in a timely and cost-efficient manner. In the last eight years the Core has generated more than 300 different lines of transgenic mice, targeted over 60 genes in mouse ES cells, produced over 120 lines of gene-targeted mice via blastocyst injection, and has cryopreserved over 100 lines of mice for UMASS researchers.

International Stem Cell Registry

StemCellThe International Stem Cell Registry (ISCR) is a freely accessible, continuously updated, searchable database for the stem cell research community. The most comprehensive database on human embryonic stem cell (hESC) and disease-specific induced pluripotent (iPS) stem cell lines, the ISCR currently provides published and validated unpublished information for over 1,500 cell lines from over 20 countries. The ISCR also includes a literature database of over 1,600 citations that are searchable by cell line name and keywords in the abstract.

Musculoskeletal Center Imaging Core

Stacey RussellDirected by Jane Lian and Ellen Gravallese, operated by Stacey Russell. The Musculoskeletal Center Imaging Core specializes in 2-D and 3-D imaging and analysis of the skeleton, biomineralized soft tissues, tumors with calcified lesions and biomaterials. A SCANO Medical MicroCT 40: Micro-Computed Tomography is used for imaging and nondestructive analysis of 3-D structure. Biomechanical properties are measured using Fracture Healing Tests and Mechanical Testing. Additional skeletal phenotyping and histomorphometry services are available.

Confocal Microscopy Core Facility

Directed and managed by Jeffrey Nickerson. The Cell Biology Three-Dimensional Microscopy Laboratory (3DML) is designed to provide excellent laser scanning confocal microscopy equipment, training, and technical support to the UMass Medical School research community. Three dimensional imaging with optical sectioning is achieved using laser lines at 476, 488, 568, and 633mm. With spectrophotometric detection, emission profiles may be determined, and overlapping spectra can be separated. There is also a detector for transmitted light. High resolution images of live cells, tissues, or embryos at very specific wavelengths can be acquired using the Live Cell Perfusion Chamber equipped with temperature control and an objective heater. Fluorescence Recovery After Photobleaching (FRAP) can be used to measure the dynamics of fluorescence labeled proteins in living cells. Time Lapse Imaging can used to present results as movies. Other modules are available to perform techniques such as Fluorescence Resonance Energy Transfer (FRET) - acceptor photobleaching for directly visualizing protein-protein interactions in living or fixed cells. Fluorescence Loss In Photobleaching (FLIP), a technique complimentary to FRAP, also used to measure molecular dynamics in living cells.

Imaging Capabilities

Charlene Baron and Tom Delaney provide photographic, web, graphic and instructional technology support and video imaging acquisition, editing and integration with research and education programs. Software training and personal tutorials are available for Adobe Photoshop, Illustrator, Acrobat, InDesign, Microsoft Office, graphic design, and image editing ethics. Poster printing, prints for publication, creation of PDF forms and other imaging services are available.

Anatomical Gift Program

Diane Person Anatomical GiftsSupervised by John Cooke, PhD, and Nancy von Hone, MS, and operated by Mike Doyle and Dianne Person. Close examination of the human body in medical schools constitutes an invaluable and indispensable part of medical education and research. Each year numbers of bodies are needed for the teaching of medical students, dental students, post graduate physicians, nurses, physiotherapists, occupational therapists and students of related disciplines. The principal source of bodies is private donation.

The Anatomical Gift Program maintains and distributes body donations to support the broad spectrum of educational and clinical research missions of UMMS. This program supports these activities in various departments, including the education of undergraduate, graduate and postgraduate students by preparing bodies, organs, tissues and cells for these purposes.

University Core Facilities

The University supports a full range of research core facilities and service centers beyond those based in the Department of Cell Biology. A complete list and individual links can be found on the Research Core Facilities website.