Fundamental changes in proteins and nucleic acids underlie many human diseases. These can be alterations in sequence, structure or modification of a critical protein (such as glycosylation or methylation), or changes in the regulation or expression levels of biological macromolecules within the cells. Human pathogens often possess unique macromolecules whose role is critical to the pathogens survival. Many recent therapeutics were discovered, using of screening and structure based drug design methodologies, through understanding the molecular mechanism by which biological macromolecules function. However, in cancer and pathogens, these discoveries can be undermined by rapid evolution causing drug resistance.
In BMP we take a molecular approach to elucidating the molecular mechanisms that underlie many human diseases. Often these approaches involve distinguishing how the disease state alters the mechanism of action of a normal human protein or level, either due to mutation or expression level. We leverage a combination of biochemistry, molecular biology, enzymology, bioinformatics, structural biology and chemical synthesis to elucidate the mechanisms that cause disease and design novel therapeutics. The diseases our faculty focus on include: congenital diseases due to mutations (including many neurodegenerative diseases such as ALS), diabetes, cancer and diseases involving changes to the immune response and infectious diseases.
The role of mutations that lead to disease are studied by: the Gilmore laboratory in oligosaccharyltransferase subunits in the family of diseases known as congenital disorders of glycosylation; the Kobertz laboratory investigates how mutations that prevent N- and O-glycosylation give rise to fatal cardiac arrhythmias; The Matthews laboratory studies mutations in Superoxide dismutase with the Zitzewitz laboratory who studies mutations in Profilin and TDP-43 all associated with ALS; The Weng laboratory, as part of the psychENCODE consortium, develop and apply computational genomic methods to study psychiatric disorders; And the Munson lab studies how mutations in endocytic regulatory proteins lead to neutrophil dysfunction, bone marrow failure, and neurological disorders.
In cancer and inflammation we are often impacted by subtle changes in the regulation and expression of key enzymes: the Kelch laboratory is studying the DNA replication and repair machinery of humans; the Royer laboratory are investigating the activation and designing of inhibitors for proteins involved in cancer (CtBP) and autoimmune disease (IRF5); the Schiffer laboratory are studying the cytidine deaminase APOBEC3 enzymes and the Thompson laboratory is developing therapeutics targeting a series of arginine modifying enzymes, including the Protein Arginine Deiminases (PADs).
In infectious diseases, the Schiffer laboratory studies the molecular basis for drug resistance and the design of new inhibitors that are less susceptible to resistance for the viral proteases: HIV, Hepatitis C, Dengue and Influenza neuraminidase. The Bolon and Zeldovich laboratories also study the evolution of drug resistance in Influenza and HIV.
Impact of Mutations:
Cancer and Inflammation:
The Royer laboratory has identified the most potent inhibitor to date against the cancer target CtBP, which contributes to a broad range of cancers. Discovered the structural basis by which IRF5 (and other family members) are activated upon phosphorylation and are developing inhibitors to disrupt the activated form, which has been linked with autoimmune diseases, particularly lupus.
| C. Robert Matthews
|| Anthony Carruthers
| Reid Gilmore
|| Brian Kelch
| Elisabet Mandon
|| Francesca Massi
|| Mary Munson
|William Royer||Celia Schiffer||Scott Shaffer|