web toolsRNA Web Tools

UMass Medical School Genomics Core
 
  Microarray technology to analyze complex genetic data. Access to Affymetrix hardware and software for Gene Chip microarray. 
UMass Medical School Flow Cytometry Core
 
 Measuring the physical and chemical characteristics of cells or other biological particles. 
UMass Medical School Deep Sequencing Core
 
 Sequencing by synthesis (MPSS) Solexa/Illumina-based technology, data management, computational analysis guidelines, on-site technical consultation.
 
UMass Center for AIDS Research (CFAR) & Molecular Biology Core
 
 Discounted reagents, DNA & RNA Oligos, Plasmids, Probes, Bioinformatics Resource, DNA fragment analysis, BiaCore SPR
 
Dharmacon 
 RNAi products and technologies: siRNA, microRNA, shRNA, qPCR assays, RNAi controls, transfections, customs synthesis, ancillary reagents, siDesign center.
 
IDT 
 Oligo design and analysis tools, tutorials.
 
Genewiz 
 DNA sequencing service. Drop box in LRB office 908.
 
The RNAi Web 
 A resource center for RNA interference (RNAi) technology.
 
RNAxs 
 A site to design potent siRNA's to knock down your gene(s) of interest. Includes all the parameters that have been shown to influence RNAi efficiency in literature.
 
RNA Modification Database
 
 A comprehensive listing of post-transcriptionally modified nuceosides form RNA.
 
RNAfold
 
 Will predict secondary structures of single stranded RNA or DNA sequences.
 
RNAhybrid
 
 For finding the minimum free energy hybridization of two RNA's.
 
The mRNA Decay Resource
 
 Sharing of information, know-how, and wisdom among researchers who study mRNA decay.
 
miRBase
 
 Searchable database of published miRNA sequences and annotation.
 
Sequence Read Archive (SRA)
 
 Stores sequencing data from the next generation of sequencing platforms including Roche 454 GS System, Illumina Genome Analyzer, Applied Biosystems SOLiD System, Helico         Heliscope, and others. 
UCSB Genome Browser
 
 The reference sequence and working draft assemblies for a large collection of genomes. Also a portal to the ENCODE project.
 
Comprehensive Transposon Database
 
 Public research domain on genetic information (DNA sequence data), as well as dissemination of databases and computer software.
 
Gene Expression Omnibus (GEO)
 
  Public functional genomics data repository supporting MIAME-compliant data submissions. 
PDB Protein Databank
 
 PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies.
 
Perl 
 Tutorial assumes a very basic knowledge of UNIX and concentrates on how to write basic working perl programs. 
Perl Regular Expressions
 
 Perl will attempt to give you 10% of regular expressions you'll use 90% of the time. 
Entrez Programming Utilities (eUtils) 
 
 Tools that provide access to Entrez data outside of the regular web query interface also helpful for retrieving search results for future use in another environment. Note: to submit large queries to the NCBI data base download in batch mode.
 
Unix Tutorials
 
 Three incredible tutorials courteously of Dr. Herve Steitz, Ph.D., former postdoc of Dr. Phillip Zamore.
 
Flybase 
 Database of Drosophila and genes and genomes.
 
Bloomington Drosphila Stock Center
 
 BDSC collects, maintains, and distributes Drosophilia melanogaster strains for research.
 
Wormbase 
 The biology and genome of C.elegens.
 
mirWIP microRNA target identification
 
 Allows you to search for targets of conserved microRNAs in C. elegans. 
Wellcome Trust Sanger Institute
 
 Rfam RNA database and web resource.
 
  
  

 



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